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Agilent technologies uls labelling ffpe dna
<t>DNA</t> extracted from <t>FFPE</t> leiomyosarcoma samples of different ages (shown in brackets) showing varied degrees of degradation, compared with commercial pooled female genomic DNA. B: Comparison of DNA extracted from paired FF and FFPE leiomyosarcoma samples (LMS 9, 10 and 11). FF samples show relatively distinct bands of high molecular weight, while corresponding FFPE samples show low molecular weight fragments in a wide range of sizes. All DNA samples are compared against a 1 Kb DNA ladder. DNA Electrophoresis was done on 1.0% agarose gels were pre-stained with Ethidium Bromide and examined under UV light.
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DNA extracted from FFPE leiomyosarcoma samples of different ages (shown in brackets) showing varied degrees of degradation, compared with commercial pooled female genomic DNA. B: Comparison of DNA extracted from paired FF and FFPE leiomyosarcoma samples (LMS 9, 10 and 11). FF samples show relatively distinct bands of high molecular weight, while corresponding FFPE samples show low molecular weight fragments in a wide range of sizes. All DNA samples are compared against a 1 Kb DNA ladder. DNA Electrophoresis was done on 1.0% agarose gels were pre-stained with Ethidium Bromide and examined under UV light.

Journal: PLoS ONE

Article Title: High Quality Genomic Copy Number Data from Archival Formalin-Fixed Paraffin-Embedded Leiomyosarcoma: Optimisation of Universal Linkage System Labelling

doi: 10.1371/journal.pone.0050415

Figure Lengend Snippet: DNA extracted from FFPE leiomyosarcoma samples of different ages (shown in brackets) showing varied degrees of degradation, compared with commercial pooled female genomic DNA. B: Comparison of DNA extracted from paired FF and FFPE leiomyosarcoma samples (LMS 9, 10 and 11). FF samples show relatively distinct bands of high molecular weight, while corresponding FFPE samples show low molecular weight fragments in a wide range of sizes. All DNA samples are compared against a 1 Kb DNA ladder. DNA Electrophoresis was done on 1.0% agarose gels were pre-stained with Ethidium Bromide and examined under UV light.

Article Snippet: FFPE tumour and reference DNA was labelled using an optimised version of the protocol for ULS labelling of FFPE DNA (Agilent®).

Techniques: Molecular Weight, Nucleic Acid Electrophoresis, Staining